single-stranded conformational polymorphism

single-stranded conformational polymorphism
(= SSCP)
Technique for detecting point mutations in genes by amplifying a region of genomic DNA (using asymmetric PCR) and running the resulting product on a high quality gel. Single base substitutions can alter the secondary structure of the fragment in the gel, producing a visible shift in its mobility.

Dictionary of molecular biology. 2004.

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